goat anti human icam 1 antibody  (R&D Systems)

Journal: PLoS ONE

Article Title: Differences in PGE 2 Production between Primary Human Monocytes and Differentiated Macrophages: Role of IL-1β and TRIF/IRF3

doi: 10.1371/journal.pone.0098517

Figure Lengend Snippet: U373-CD14 cells were transfected with control siRNA or with siRNA targeting TRIF or IRF3 (A, C), or TRAM (B, D). Transfected cells were left untreated (solid bars), or were incubated with LPS at 1 ng/ml (shaded bars), with rIL-1β at 100 ng/ml for 18 h (striped bars), or were primed for 1 h with LPS and incubated with rIL-1β for additional 18 h (open bars). Cells were collected and COX-2 mRNA (A, B) and mPGES-1 mRNA (C, D) expressions were assayed by qPCR. The data are shown as fold increase over control PCR with primers specific to β-actin , run in the same samples. PCR was performed using triplicate wells and the data are shown as mean fold increase ± STDEV. This is representative of three experiments.

Article Snippet: For the Western Blotting of the cell lysates prepared from transfected cells and resolved in SDS-PAGE, the following antibodies were used: goat anti-human TRIF Ab (Cat AF6216, R&D Systems), rabbit anti-human TRAM Ab (Cat ab17221, Abcam), and rabbit anti-human IRF3 Ab (Cat 4302, Cell Signaling).

Techniques: Transfection, Control, Incubation